Using Laboratory DNA Testing to Confirm Ancestry

Nov 16, 2021 | Relationship

Using Laboratory DNA Tests to Confirm Ancestry

Once you’ve located unknown family members, wouldn’t you like to know for sure if you’re biologically related to them? Researching your family tree takes a lot of time and is associated with costs.  Rest assured, STR laboratory DNA testing can confirm with certainty if ancestry information uncovered during research is true. It’s a highly-accurate method for expanding your family tree. 


DNA Tests for Ancestry

DNA tests that allow public sharing of genetic data can provide vital information about biological relationships between potential living relatives, making the test an invaluable research tool for people interested in tracing their genealogy or ancestry. The results of DNA tests can link common ancestors and answer otherwise unanswered questions about a person’s family tree. The results of these types of DNA tests can help you find previously-unknown family members, and in some cases may reveal an unexpected truth about a paternal or maternal tie.  Since the popularity of ancestry exploded, DDC has seen a marked increase in the number of people seeking to confirm possible biological relationships discovered during ancestry testing. 

Accredited laboratories like DDC offer many STR-based laboratory DNA testing options that help confirm questions about possible family relationships, including:

  • Maternity: While similar to a DNA paternity test, this analysis compares a child’s DNA profile with their mother’s
  • Grandparent: When the father isn’t available for a paternity test, this test can determine the statistical probability of whether or not the alleged grandmother and/or grandfather is biologically related to tested children
  • Sibling: This test determines whether two individuals share one or both parents. Although an indirect way to prove or disprove paternity, it’s useful when the alleged fathercan’t be tested.
  • Aunt-Uncle: Called avuncular testing, this is a good option when the father isn’t available for testing, because uncles and aunts share 25% of variable DNA with their nieces and nephews.
  • Family reconstruction: This type of testing involves gathering DNA samples from secondary sources in order to construct a DNA profile when direct samples aren’t available. For example, the child’s DNA profile is compared with DNA profiles of at least two of the potential father’s close relatives (e.g. a full sibling or biological parent). If only two relatives are available, it’s recommended that the mother participates to help strengthen results 
  • Male Lineage (Y-STR): See below
  • Female Lineage (mtDNA): See below 
  • Twins (Twin Zygosity): This DNA analysis definitively shows whether twins are identical or fraternal. This test can be helpful for establishing genetic compatibility for blood or tissue donation or determining if both twins inherited genetic defects.



Watch how a sibling test changed one man’s life >>


DNA Testing Technology: Y-STR and mtDNA

Due to advances in DNA testing technology, tests can provide definitive answers about a person’s ancestry. In addition to other forms of technology, accredited laboratories use a combination of two types of tests;the Y-STR Paternal Lineage Test and the mtDNA Maternal Lineage Test are useful for determining lines of ancestry to establish or rule out relationships between more distant relatives. 

Laboratory DNA Testing Using Y-STR Analysis

The Y-STR test is used to determine whether or not two or more males are related through their fathers via the paternal/male line (e.g. brothers, paternal cousins, grandfather/grandson, uncle/nephew, etc.). The results of this test are often used to provide additional evidence in paternity situations in which the possible father is not available for testing. The information carried on the Y chromosome remains relatively unchanged throughout generations. Because the Y chromosome follows the same father-to-son pattern as surnames in Western culture, this test is sometimes called the “Surname Test.” A tangible timekeeper of history, the Y chromosome enables:

  • Tracing human evolution
  • Tracking migration patterns
  • Determining relatedness in groups of people
  • Answering paternity questions going back generations

Although Y-STR analysis cannot distinguish between males who belong to the same paternal line, it’s useful for excluding males from a possible biological relationship. For example, if a male child and his possible paternal uncle are tested, their Y-STR profiles must match in order to determine a biological familial relationship. If they don’t match, the potential uncle is excluded as a biological relative of the child.

NOTE: Because women do have a Y chromosome, individuals born as females cannot do this test. However they can ask their brother or father to test.

Laboratory DNA Testing Using mtDNA Analysis

Mitochondrial DNA (mtDNA) is inherited through the maternal line throughout time, essentially unchanged. Unlike the Y chromosome that can only be passed down from father to son, females inherit mtDNA from their mother and pass on mtDNA to both their male and female children. Since mtDNA is maternally inherited, results from testing enable researchers to trace lineage far back in time. The mtDNA test shows the origin of maternal ancestors as well as individuals you’re related to in the direct maternal line. Minor mutations that occur in mtDNA throughout generations make certain regions of mtDNA unique to a family line. 

Again, while the Y-STR test can only be performed on males, the mtDNA test is applicable to both males and females. Because everyone from the same maternal line has similar mtDNA sequences, this test is an accurate method for establishing connections along the mother’s familial line.

Not Sure Where to Start? Speak with DNA Diagnostics Center

Since every situation is unique, we highly recommend that you call our paternity experts at 1.800.929.0847 to ensure you order the test that’s right for you. We’re committed to helping you find the answers you need, and stand behind our promise of making the testing process convenient, confidential, accurate, and fast. 

Call us for a FREE confidential consultation at 800-929-0847: We’re here to help.

Do you have questions or comments about this topic? Share in the comments and we’ll answer.

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  1. Vivian

    I was contacted and told that family friends have reason to believe that my father is the father of 2 siblings. If i give them a DNA sample will that help them to find out the truth as to know if my father is also their father?

    • DDC

      Hi, Vivian. Yes, it can. Although with a half-sibling test you’re more likely to get conclusive results if the mothers are able and willing to participate. Of course, the ultimate best scenario is for them to test with your father, if he’s living and willing.

      • Katherine

        Hi do you have any offices in Durban South Africa

        I am needing to prove that a British Father is the father of the South African child..

        The child is in SA and the father is in the uk how do we go about thus and what is the procedure and cost

        • DDC

          Hi, Katherine. Yes, please contact our corporate partner in South Africa via this site:

  2. Barbara

    I’ve wanted to see what kind of trible blood I have can DNA
    Help see what type of bloodline I come from

    • DDC

      Hi, Barbara. There is no DNA test that can tell you what Native-American tribe you may or not be part of. Sorry!


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