DNA Testing Technology

DDC Medical, a division of DNA Diagnostics Center, offers human cell line identification and mycoplasma detection services. Studies have found an alarming percentage of human cell lines purchased from commercial repositories can be contaminated or misidentified. In addition, genetic instability of some cell lines can result in changes at the DNA level that can compromise research results. Articles published on these topics indicate as much as 20 to 33 percent of all cell lines are misidentified or contaminated, but there is a simple and affordable solution.

Peer review journals throughout the research community recommends cell line Identification and mycoplasma detection as part of the publication process. Grant providers are also aware of the issue and requiring cell line identification and mycoplasma detection ensures a sound investment.

Who might be interested in this test?

• Academic researchers and teaching labs
• Pharma labs and researchers
• Biotech labs and researchers
• Government labs and researchers
• Other  private and non-profit cancer and cell-based research institutions

When should cell line identification be completed?

• When a new line is first established or purchased
• As a control measure at the start of a new study and concluding a study
• At least once every 6 months in active culture
• Before freezing down for storage
• When an unexpected result occurs

Cell Line Identification and Mycoplasma Detection

STR Analysis for Identification

The DNA profile generated for cell line authentication utilizes STR (Short Tandem Repeat) analysis of a number of highly polymorphic unlinked loci. Each locus is characterized by repeat units typically 4 bases in length. The number of repeats detected in a DNA fragment identifies each allele. For example, if the designation at the D5S818 locus is 14, 16, two DNA fragments were detected; one with 14 repeats and one with 16 repeats. The loci used for cell line authentication are utilized for human identification and are not designed to detect non-human DNA.

Mycoplasma Detection

Unlike most bacterial and fungal contaminants, which are easily detected by changes in visual appearance of the culture, mycoplasma contamination cannot be detected without further analysis, which can alter a cell line’s growth pattern, and in some cases, lead to the death of the cell line.

To rule out mycoplasma in your cell cultures, DDC Medical offers a DNA PCR detection test that will screen for 19 species, including the 6 most common contaminants found in cell cultures. DDC Medical tests for the most common contaminants and 95% of all mycoplasma contaminants.

For more about Cell Line Identification, call us at 0845 408 2084